interleukin 1 receptor antagonist (MedChemExpress)
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Interleukin 1 Receptor Antagonist, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/interleukin+1+receptor+antagonist/Recombinant+Human+Interleukin-1+Receptor+Antagonist+Protein/pm41013704-59-12-17
Average 93 stars, based on 4 article reviews
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Blocking Assay:Article Title: The Glycolysis-HIF-1α axis induces IL-1β of macrophages in rheumatoid arthritis Article Snippet: For cytokines stimulation, HMDMs were incubated with 50ng/ml Tumor necrosis factor alpha (TNF-α) (Peprotech, 300–01 A), 50ng/ml Interleukin-1 beta (IL-1β) (Peprotech, 200-01B), 50ng/ml Granulocyte-macrophage colony stimulating factor (GM-CSF) (Novoprotein, C003), 50ng/ml Interleukin-6 (IL-6) (Peprotech, 200-06) and 50ng/ml IFN-γ (Novoprotein, CI57) for 24 h. For inhibition of glycolysis, HMDMs were pretreated with 10 μM or 40 μM of 3-Bromopyruvic acid (3BrPA, HY-19992, MedChemExpress), 5mM of 2-Deoxy-D-glucose (2-DG, MedChemExpress, HY-13966) for 3 h. For stabilization and inhibition of HIF-1α, HMDMs were pretreated with 100 μM of Dimethyloxallyl Glycine (DMOG, MedChemExpress, HY-15893) and 20 μM of LW6 (HY-13671, MedChemExpress) for 3 h, respectively. .. For cytokine blocking, HMDMs were incubated with RA serum with 100ng/ml of Article Title: The Glycolysis-HIF-1α axis induces IL-1β of macrophages in rheumatoid arthritis. Article Snippet: For cytokines stimulation, HMDMs were incubated with 50ng/ml Tumor necrosis factor alpha (TNF-α) (Peprotech, 300–01 A), 50ng/ml Interleukin-1 beta (IL-1β) (Peprotech, 200-01B), 50ng/ml Granulocyte-macrophage colony stimulating factor (GM-CSF) (Novoprotein, C003), 50ng/ml Interleukin-6 (IL-6) (Peprotech, 200-06) and 50ng/ml IFN-γ (Novoprotein, CI57) for 24 h. For inhibition of glycolysis, HMDMs were pretreated with 10 μM or 40 μM of 3-Bromopyruvic acid (3BrPA, HY-19992, MedChemExpress), 5mM of 2-Deoxy-D-glucose (2-DG, MedChemExpress, HY-13966) for 3 h. For stabilization and inhibition of HIF-1α, HMDMs were pretreated with 100 μM of Dimethyloxallyl Glycine (DMOG, MedChemExpress, HY-15893) and 20 μM of LW6 (HY-13671, MedChemExpress) for 3 h, respectively. .. For cytokine blocking, HMDMs were incubated with RA serum with 100ng/ml of Incubation:Article Title: The Glycolysis-HIF-1α axis induces IL-1β of macrophages in rheumatoid arthritis Article Snippet: For cytokines stimulation, HMDMs were incubated with 50ng/ml Tumor necrosis factor alpha (TNF-α) (Peprotech, 300–01 A), 50ng/ml Interleukin-1 beta (IL-1β) (Peprotech, 200-01B), 50ng/ml Granulocyte-macrophage colony stimulating factor (GM-CSF) (Novoprotein, C003), 50ng/ml Interleukin-6 (IL-6) (Peprotech, 200-06) and 50ng/ml IFN-γ (Novoprotein, CI57) for 24 h. For inhibition of glycolysis, HMDMs were pretreated with 10 μM or 40 μM of 3-Bromopyruvic acid (3BrPA, HY-19992, MedChemExpress), 5mM of 2-Deoxy-D-glucose (2-DG, MedChemExpress, HY-13966) for 3 h. For stabilization and inhibition of HIF-1α, HMDMs were pretreated with 100 μM of Dimethyloxallyl Glycine (DMOG, MedChemExpress, HY-15893) and 20 μM of LW6 (HY-13671, MedChemExpress) for 3 h, respectively. .. For cytokine blocking, HMDMs were incubated with RA serum with 100ng/ml of Article Title: The Glycolysis-HIF-1α axis induces IL-1β of macrophages in rheumatoid arthritis. Article Snippet: For cytokines stimulation, HMDMs were incubated with 50ng/ml Tumor necrosis factor alpha (TNF-α) (Peprotech, 300–01 A), 50ng/ml Interleukin-1 beta (IL-1β) (Peprotech, 200-01B), 50ng/ml Granulocyte-macrophage colony stimulating factor (GM-CSF) (Novoprotein, C003), 50ng/ml Interleukin-6 (IL-6) (Peprotech, 200-06) and 50ng/ml IFN-γ (Novoprotein, CI57) for 24 h. For inhibition of glycolysis, HMDMs were pretreated with 10 μM or 40 μM of 3-Bromopyruvic acid (3BrPA, HY-19992, MedChemExpress), 5mM of 2-Deoxy-D-glucose (2-DG, MedChemExpress, HY-13966) for 3 h. For stabilization and inhibition of HIF-1α, HMDMs were pretreated with 100 μM of Dimethyloxallyl Glycine (DMOG, MedChemExpress, HY-15893) and 20 μM of LW6 (HY-13671, MedChemExpress) for 3 h, respectively. .. For cytokine blocking, HMDMs were incubated with RA serum with 100ng/ml of Concentration Assay:Article Title: ER-mitochondria association negatively affects wound healing by regulating NLRP3 activation Article Snippet: For experiments with NLRP3 Inhibitor, MCC950 (Sigma-Aldrich) was diluted into the medium at the final concentration of 10 μM. .. For experiments with Article Title: ER-mitochondria association negatively affects wound healing by regulating NLRP3 activation. Article Snippet: For experiments with NLRP3 Inhibitor, MCC950 (Sigma-Aldrich) was diluted into the medium at the final concentration of 10 μM. .. For experiments with |
