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interleukin 1 receptor antagonist  (MedChemExpress)


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    Structured Review

    MedChemExpress interleukin 1 receptor antagonist
    Interleukin 1 Receptor Antagonist, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/interleukin+1+receptor+antagonist/Recombinant+Human+Interleukin-1+Receptor+Antagonist+Protein/pm41013704-59-12-17
    Average 93 stars, based on 4 article reviews
    interleukin 1 receptor antagonist - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: The Glycolysis-HIF-1α axis induces IL-1β of macrophages in rheumatoid arthritis
    Article Snippet: For cytokines stimulation, HMDMs were incubated with 50ng/ml Tumor necrosis factor alpha (TNF-α) (Peprotech, 300–01 A), 50ng/ml Interleukin-1 beta (IL-1β) (Peprotech, 200-01B), 50ng/ml Granulocyte-macrophage colony stimulating factor (GM-CSF) (Novoprotein, C003), 50ng/ml Interleukin-6 (IL-6) (Peprotech, 200-06) and 50ng/ml IFN-γ (Novoprotein, CI57) for 24 h. For inhibition of glycolysis, HMDMs were pretreated with 10 μM or 40 μM of 3-Bromopyruvic acid (3BrPA, HY-19992, MedChemExpress), 5mM of 2-Deoxy-D-glucose (2-DG, MedChemExpress, HY-13966) for 3 h. For stabilization and inhibition of HIF-1α, HMDMs were pretreated with 100 μM of Dimethyloxallyl Glycine (DMOG, MedChemExpress, HY-15893) and 20 μM of LW6 (HY-13671, MedChemExpress) for 3 h, respectively. .. For cytokine blocking, HMDMs were incubated with RA serum with 100ng/ml of Interleukin-1 receptor antagonist (IL-1RN, HY-P7029, MedChemExpress), 10 μg/ml of Infliximab (HY-P9970, MedChemExpress), or Lenzilumab (HY-P99207, MedChemExpress) for 24 h, respectively. .. HMDMs were detached from cell culture plates with 0.25% trypsin (Thermo, 25200072) and incubated at 37 °C in a 5% CO2 incubator for 5 min, with the digestion process repeated twice.

    Article Title: The Glycolysis-HIF-1α axis induces IL-1β of macrophages in rheumatoid arthritis.
    Article Snippet: For cytokines stimulation, HMDMs were incubated with 50ng/ml Tumor necrosis factor alpha (TNF-α) (Peprotech, 300–01 A), 50ng/ml Interleukin-1 beta (IL-1β) (Peprotech, 200-01B), 50ng/ml Granulocyte-macrophage colony stimulating factor (GM-CSF) (Novoprotein, C003), 50ng/ml Interleukin-6 (IL-6) (Peprotech, 200-06) and 50ng/ml IFN-γ (Novoprotein, CI57) for 24 h. For inhibition of glycolysis, HMDMs were pretreated with 10 μM or 40 μM of 3-Bromopyruvic acid (3BrPA, HY-19992, MedChemExpress), 5mM of 2-Deoxy-D-glucose (2-DG, MedChemExpress, HY-13966) for 3 h. For stabilization and inhibition of HIF-1α, HMDMs were pretreated with 100 μM of Dimethyloxallyl Glycine (DMOG, MedChemExpress, HY-15893) and 20 μM of LW6 (HY-13671, MedChemExpress) for 3 h, respectively. .. For cytokine blocking, HMDMs were incubated with RA serum with 100ng/ml of Interleukin-1 receptor antagonist (IL-1RN, HY-P7029, MedChemExpress), 10 μg/ml of Infliximab (HY-P9970, MedChemExpress), or Lenzilumab (HY-P99207, MedChemExpress) for 24 h, respectively. .. HMDMs were detached from cell culture plates with 0.25% trypsin (Thermo, 25200072) and incubated at 37 °C in a 5% CO2 incubator for 5 min, with the digestion process repeated twice.

    Incubation:

    Article Title: The Glycolysis-HIF-1α axis induces IL-1β of macrophages in rheumatoid arthritis
    Article Snippet: For cytokines stimulation, HMDMs were incubated with 50ng/ml Tumor necrosis factor alpha (TNF-α) (Peprotech, 300–01 A), 50ng/ml Interleukin-1 beta (IL-1β) (Peprotech, 200-01B), 50ng/ml Granulocyte-macrophage colony stimulating factor (GM-CSF) (Novoprotein, C003), 50ng/ml Interleukin-6 (IL-6) (Peprotech, 200-06) and 50ng/ml IFN-γ (Novoprotein, CI57) for 24 h. For inhibition of glycolysis, HMDMs were pretreated with 10 μM or 40 μM of 3-Bromopyruvic acid (3BrPA, HY-19992, MedChemExpress), 5mM of 2-Deoxy-D-glucose (2-DG, MedChemExpress, HY-13966) for 3 h. For stabilization and inhibition of HIF-1α, HMDMs were pretreated with 100 μM of Dimethyloxallyl Glycine (DMOG, MedChemExpress, HY-15893) and 20 μM of LW6 (HY-13671, MedChemExpress) for 3 h, respectively. .. For cytokine blocking, HMDMs were incubated with RA serum with 100ng/ml of Interleukin-1 receptor antagonist (IL-1RN, HY-P7029, MedChemExpress), 10 μg/ml of Infliximab (HY-P9970, MedChemExpress), or Lenzilumab (HY-P99207, MedChemExpress) for 24 h, respectively. .. HMDMs were detached from cell culture plates with 0.25% trypsin (Thermo, 25200072) and incubated at 37 °C in a 5% CO2 incubator for 5 min, with the digestion process repeated twice.

    Article Title: The Glycolysis-HIF-1α axis induces IL-1β of macrophages in rheumatoid arthritis.
    Article Snippet: For cytokines stimulation, HMDMs were incubated with 50ng/ml Tumor necrosis factor alpha (TNF-α) (Peprotech, 300–01 A), 50ng/ml Interleukin-1 beta (IL-1β) (Peprotech, 200-01B), 50ng/ml Granulocyte-macrophage colony stimulating factor (GM-CSF) (Novoprotein, C003), 50ng/ml Interleukin-6 (IL-6) (Peprotech, 200-06) and 50ng/ml IFN-γ (Novoprotein, CI57) for 24 h. For inhibition of glycolysis, HMDMs were pretreated with 10 μM or 40 μM of 3-Bromopyruvic acid (3BrPA, HY-19992, MedChemExpress), 5mM of 2-Deoxy-D-glucose (2-DG, MedChemExpress, HY-13966) for 3 h. For stabilization and inhibition of HIF-1α, HMDMs were pretreated with 100 μM of Dimethyloxallyl Glycine (DMOG, MedChemExpress, HY-15893) and 20 μM of LW6 (HY-13671, MedChemExpress) for 3 h, respectively. .. For cytokine blocking, HMDMs were incubated with RA serum with 100ng/ml of Interleukin-1 receptor antagonist (IL-1RN, HY-P7029, MedChemExpress), 10 μg/ml of Infliximab (HY-P9970, MedChemExpress), or Lenzilumab (HY-P99207, MedChemExpress) for 24 h, respectively. .. HMDMs were detached from cell culture plates with 0.25% trypsin (Thermo, 25200072) and incubated at 37 °C in a 5% CO2 incubator for 5 min, with the digestion process repeated twice.

    Concentration Assay:

    Article Title: ER-mitochondria association negatively affects wound healing by regulating NLRP3 activation
    Article Snippet: For experiments with NLRP3 Inhibitor, MCC950 (Sigma-Aldrich) was diluted into the medium at the final concentration of 10 μM. .. For experiments with Interleukin-1 Receptor Antagonist, Anakinra (MedChemExpress, Monmouth Junction, NJ, USA) was used at the final concentration of 5 μg/ml. .. Cells were seeded in 6-well plates, treated as indicated above, and then lysed in Denaturing Lysis Buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, 0.1% Sodium Dodecyl Sulfate), supplemented with 1 mM PMSF, protease inhibitors (Sigma-Aldrich) and PhosStop (Roche, Basil, Switzerland).

    Article Title: ER-mitochondria association negatively affects wound healing by regulating NLRP3 activation.
    Article Snippet: For experiments with NLRP3 Inhibitor, MCC950 (Sigma-Aldrich) was diluted into the medium at the final concentration of 10 μM. .. For experiments with Interleukin-1 Receptor Antagonist, Anakinra (MedChemExpress, Monmouth Junction, NJ, USA) was used at the final concentration of 5 μg/ml. .. Cells were seeded in 6-well plates, treated as indicated above, and then lysed in Denaturing Lysis Buffer (50mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 0.1% Sodium Dodecyl Sulfate), supplemented with 1 mM PMSF, protease inhibitors (Sigma-Aldrich) and PhosStop (Roche, Basil, Switzerland).



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    GCDCA-mediated hepatocyte IL-1β secretion activates HSCs. ( A ) Western blot analysis ofα-SMA and Collagen-I in LX2 cells treated with conditioned medium (CM) from GCDCA/LPS-stimulated L02 hepatocytes. ( B ) The protein levels of α-SMA and Collagen-I in LX2 cells with treatment of IL-1 receptor antagonist (IL-1RA, 200 ng/mL) upon CM exposure. ( C and D ) Representative images and quantification of LX2 migration upon CM exposure detected by wound healing assay. ( E ) CCK-8 assay of LX2 proliferation by CM treatment. n ≥ 3 for each group. a P < 0.05.

    Journal: Journal of Inflammation Research

    Article Title: Glycochenodeoxycholic acid induces the release of IL-1beta from L02 cells via the NLRP3/caspase-1/GSDMD pathway to activate LX2 cells

    doi: 10.2147/JIR.S532042

    Figure Lengend Snippet: GCDCA-mediated hepatocyte IL-1β secretion activates HSCs. ( A ) Western blot analysis ofα-SMA and Collagen-I in LX2 cells treated with conditioned medium (CM) from GCDCA/LPS-stimulated L02 hepatocytes. ( B ) The protein levels of α-SMA and Collagen-I in LX2 cells with treatment of IL-1 receptor antagonist (IL-1RA, 200 ng/mL) upon CM exposure. ( C and D ) Representative images and quantification of LX2 migration upon CM exposure detected by wound healing assay. ( E ) CCK-8 assay of LX2 proliferation by CM treatment. n ≥ 3 for each group. a P < 0.05.

    Article Snippet: Conditioned media from treated L02 cells were co-cultured with LX2 cells in the presence/absence of Interleukin-1 receptor antagonist (IL-1RA) (200 ng/mL, HY-P7029A, MCE).

    Techniques: Western Blot, Migration, Wound Healing Assay, CCK-8 Assay